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such as fiber in meat)", "foodNutrientSource": { "id": 5, "code": "7", "description": "Assumed zero" } }, "id": 1486681, "amount": 0.0, "dataPoints": 0 }, { "type": "FoodNutrient", "nutrient": { "id": 1246, "number": "578", "name": "Vitamin B-12, added", "rank": 7340, "unitName": "\u00b5g" }, "foodNutrientDerivation": { "id": 68, "code": "Z", "description": "Assumed zero (Insignificant amount or not naturally occurring in a food, such as fiber in meat)", "foodNutrientSource": { "id": 5, "code": "7", "description": "Assumed zero" } }, "id": 1486683, "amount": 0.0, "dataPoints": 0 }, { "type": "FoodNutrient", "nutrient": { "id": 1106, "number": "320", "name": "Vitamin A, RAE", "rank": 7420, "unitName": "\u00b5g" }, "foodNutrientDerivation": { "id": 49, "code": "NC", "description": "Calculated", "foodNutrientSource": { "id": 2, "code": "4", "description": "Calculated or imputed" } }, "id": 1486674, "amount": 0.0, "dataPoints": 0 }, { "type": "FoodNutrient", "nutrient": { "id": 1105, "number": "319", "name": "Retinol", "rank": 7430, "unitName": "\u00b5g" }, "foodNutrientDerivation": { "id": 68, "code": "Z", "description": "Assumed zero (Insignificant amount or not naturally occurring in a food, such as fiber in meat)", "foodNutrientSource": { "id": 5, "code": "7", "description": "Assumed zero" } }, "id": 1486673, "amount": 0.0, "dataPoints": 0 }, { "type": "FoodNutrient", "nutrient": { "id": 1107, "number": "321", "name": "Carotene, beta", "rank": 7440, "unitName": "\u00b5g" }, "foodNutrientDerivation": { "id": 16, "code": "BFSN", "description": "Based on another form of the food or similar food; Concentration adjustment; Solids; Retention factors not used", "foodNutrientSource": { "id": 2, "code": "4", "description": "Calculated or imputed" } }, "id": 1486671, "amount": 1.0, "dataPoints": 0 }, { "type": "FoodNutrient", "nutrient": { "id": 1108, "number": "322", "name": "Carotene, alpha", "rank": 7450, "unitName": "\u00b5g" }, "foodNutrientDerivation": { "id": 16, "code": "BFSN", "description": "Based on another form of the food or similar food; Concentration adjustment; Solids; Retention factors not used", "foodNutrientSource": { "id": 2, "code": "4", "description": "Calculated or imputed" } }, "id": 1486672, "amount": 0.0, "dataPoints": 0 }, { "type": "FoodNutrient", "nutrient": { "id": 1120, "number": "334", "name": "Cryptoxanthin, beta", "rank": 7460, "unitName": "\u00b5g" }, "foodNutrientDerivation": { "id": 16, "code": "BFSN", "description": "Based on another form of the food or similar food; Concentration adjustment; Solids; Retention factors not used", "foodNutrientSource": { "id": 2, "code": "4", "description": "Calculated or imputed" } }, "id": 1486620, "amount": 0.0, "dataPoints": 0 }, { "type": "FoodNutrient", "nutrient": { "id": 1104, "number": "318", "name": "Vitamin A, IU", "rank": 7500, "unitName": "IU" }, "foodNutrientDerivation": { "id": 49, "code": "NC", "description": "Calculated", "foodNutrientSource": { "id": 2, "code": "4", "description": "Calculated or imputed" } }, "id": 1486675, "amount": 2.0, "dataPoints": 0 }, { "type": "FoodNutrient", "nutrient": { "id": 1122, "number": "337", "name": "Lycopene", "rank": 7530, "unitName": "\u00b5g" }, "foodNutrientDerivation": { "id": 16, "code": "BFSN", "description": "Based on another form of the food or similar food; Concentration adjustment; Solids; Retention factors not used", "foodNutrientSource": { "id": 2, "code": "4", "description": "Calculated or imputed" } }, "id": 1486621, "amount": 0.0, "dataPoints": 0 }, { "type": "FoodNutrient", "nutrient": { "id": 1123, "number": "338", "name": "Lutein + zeaxanthin", "rank": 7560, "unitName": "\u00b5g" }, "foodNutrientDerivation": { "id": 16, "code": "BFSN", "description": "Based on another form of the food or similar food; Concentration adjustment; Solids; Retention factors not used", "foodNutrientSource": { "id": 2, "code": "4", "description": "Calculated or imputed" } }, "id": 1486597, "amount": 4.0, 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"FoodNutrient", "nutrient": { "id": 1125, "number": "341", "name": "Tocopherol, beta", "rank": 8000, "unitName": "mg" }, "foodNutrientDerivation": { "id": 43, "code": "JO", "description": "Aggregated data involving combinations of data with different source codes when at least one code is not 1, 6, 12, or 13", "foodNutrientSource": { "id": 8, "code": "11", "description": "Aggregated data involving comb. of codes other then 1,12 or6" } }, "id": 1486598, "amount": 0.0, "dataPoints": 5, "max": 0.0, "min": 0.0 }, { "type": "FoodNutrient", "nutrient": { "id": 1126, "number": "342", "name": "Tocopherol, gamma", "rank": 8100, "unitName": "mg" }, "foodNutrientDerivation": { "id": 43, "code": "JO", "description": "Aggregated data involving combinations of data with different source codes when at least one code is not 1, 6, 12, or 13", "foodNutrientSource": { "id": 8, "code": "11", "description": "Aggregated data involving comb. of codes other then 1,12 or6" } }, "id": 1486599, "amount": 0.0, 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56, "code": "PIK", "description": "Based on physical composition; Derived from imputed data; Known physical composition", "foodNutrientSource": { "id": 2, "code": "4", "description": "Calculated or imputed" } }, "id": 1582478, "amount": 0.0, "dataPoints": 0 }, { "type": "FoodNutrient", "nutrient": { "id": 1165, "number": "404", "name": "Thiamin", "rank": 6400, "unitName": "mg" }, "foodNutrientDerivation": { "id": 54, "code": "PAK", "description": "Based on physical composition; Derived from analytical data; Known physical composition", "foodNutrientSource": { "id": 1, "code": "1", "description": "Analytical or derived from analytical" } }, "id": 1582479, "amount": 0.078, "dataPoints": 0 }, { "type": "FoodNutrient", "nutrient": { "id": 1166, "number": "405", "name": "Riboflavin", "rank": 6500, "unitName": "mg" }, "foodNutrientDerivation": { "id": 54, "code": "PAK", "description": "Based on physical composition; Derived from analytical data; Known physical composition", 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"number": "320", "name": "Vitamin A, RAE", "rank": 7420, "unitName": "\u00b5g" }, "foodNutrientDerivation": { "id": 54, "code": "PAK", "description": "Based on physical composition; Derived from analytical data; Known physical composition", "foodNutrientSource": { "id": 1, "code": "1", "description": "Analytical or derived from analytical" } }, "id": 1582470, "amount": 12.0, "dataPoints": 0 }, { "type": "FoodNutrient", "nutrient": { "id": 1105, "number": "319", "name": "Retinol", "rank": 7430, "unitName": "\u00b5g" }, "foodNutrientDerivation": { "id": 54, "code": "PAK", "description": "Based on physical composition; Derived from analytical data; Known physical composition", "foodNutrientSource": { "id": 1, "code": "1", "description": "Analytical or derived from analytical" } }, "id": 1582469, "amount": 12.0, "dataPoints": 0 }, { "type": "FoodNutrient", "nutrient": { "id": 1107, "number": "321", "name": "Carotene, beta", "rank": 7440, "unitName": "\u00b5g" }, "foodNutrientDerivation": { "id": 54, "code": "PAK", "description": "Based on physical composition; Derived from analytical data; Known physical composition", "foodNutrientSource": { "id": 1, "code": "1", "description": "Analytical or derived from analytical" } }, "id": 1582471, "amount": 0.0, "dataPoints": 0 }, { "type": "FoodNutrient", "nutrient": { "id": 1108, "number": "322", "name": "Carotene, alpha", "rank": 7450, "unitName": "\u00b5g" }, "foodNutrientDerivation": { "id": 54, "code": "PAK", "description": "Based on physical composition; Derived from analytical data; Known physical composition", "foodNutrientSource": { "id": 1, "code": "1", "description": "Analytical or derived from analytical" } }, "id": 1582472, "amount": 0.0, "dataPoints": 0 }, { "type": "FoodNutrient", "nutrient": { "id": 1120, "number": "334", "name": "Cryptoxanthin, beta", "rank": 7460, "unitName": "\u00b5g" }, "foodNutrientDerivation": { "id": 54, "code": "PAK", "description": "Based on physical composition; Derived from analytical 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{ "id": 54, "code": "PAK", "description": "Based on physical composition; Derived from analytical data; Known physical composition", "foodNutrientSource": { "id": 1, "code": "1", "description": "Analytical or derived from analytical" } }, "id": 1582530, "amount": 0.0, "dataPoints": 0 }, { "type": "FoodNutrient", "nutrient": { "id": 1273, "number": "624", "name": "SFA 22:0", "rank": 11200, "unitName": "g" }, "foodNutrientDerivation": { "id": 54, "code": "PAK", "description": "Based on physical composition; Derived from analytical data; Known physical composition", "foodNutrientSource": { "id": 1, "code": "1", "description": "Analytical or derived from analytical" } }, "id": 1582504, "amount": 0.0, "dataPoints": 0 }, { "type": "FoodNutrient", "nutrient": { "id": 1301, "number": "654", "name": "SFA 24:0", "rank": 11300, "unitName": "g" }, "foodNutrientDerivation": { "id": 54, "code": "PAK", "description": "Based on physical composition; Derived from analytical data; Known physical 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0.064, "dataPoints": 0 }, { "type": "FoodNutrient", "nutrient": { "id": 1276, "number": "627", "name": "PUFA 18:4", "rank": 14250, "unitName": "g" }, "foodNutrientDerivation": { "id": 56, "code": "PIK", "description": "Based on physical composition; Derived from imputed data; Known physical composition", "foodNutrientSource": { "id": 2, "code": "4", "description": "Calculated or imputed" } }, "id": 1582535, "amount": 0.0, "dataPoints": 0 }, { "type": "FoodNutrient", "nutrient": { "id": 1313, "number": "672", "name": "PUFA 20:2 n-6 c,c", "rank": 14300, "unitName": "g" }, "foodNutrientDerivation": { "id": 54, "code": "PAK", "description": "Based on physical composition; Derived from analytical data; Known physical composition", "foodNutrientSource": { "id": 1, "code": "1", "description": "Analytical or derived from analytical" } }, "id": 1582541, "amount": 0.009, "dataPoints": 0 }, { "type": "FoodNutrient", "nutrient": { "id": 1325, "number": "689", "name": "PUFA 20:3", "rank": 14400, 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"code": "1", "description": "Analytical or derived from analytical" } }, "id": 1582503, "amount": 0.0, "dataPoints": 0 }, { "type": "FoodNutrient", "nutrient": { "id": 1257, "number": "605", "name": "Fatty acids, total trans", "rank": 15400, "unitName": "g" }, "foodNutrientDerivation": { "id": 56, "code": "PIK", "description": "Based on physical composition; Derived from imputed data; Known physical composition", "foodNutrientSource": { "id": 2, "code": "4", "description": "Calculated or imputed" } }, "id": 1582498, "amount": 0.099, "dataPoints": 0 }, { "type": "FoodNutrient", "nutrient": { "id": 1253, "number": "601", "name": "Cholesterol", "rank": 15700, "unitName": "mg" }, "foodNutrientDerivation": { "id": 54, "code": "PAK", "description": "Based on physical composition; Derived from analytical data; Known physical composition", "foodNutrientSource": { "id": 1, "code": "1", "description": "Analytical or derived from analytical" } }, "id": 1582497, "amount": 96.0, "dataPoints": 0 }, { "nutrient": { "id": 2042, "number": "500", "name": "Amino acids", "rank": 16250, "unitName": "g" }, "type": "FoodNutrient" }, { "type": "FoodNutrient", "nutrient": { "id": 1210, "number": "501", "name": "Tryptophan", "rank": 16300, "unitName": "g" }, "foodNutrientDerivation": { "id": 54, "code": "PAK", "description": "Based on physical composition; Derived from analytical data; Known physical composition", "foodNutrientSource": { "id": 1, "code": "1", "description": "Analytical or derived from analytical" } }, "id": 1582486, "amount": 0.318, "dataPoints": 0 }, { "type": "FoodNutrient", "nutrient": { "id": 1211, "number": "502", "name": "Threonine", "rank": 16400, "unitName": "g" }, "foodNutrientDerivation": { "id": 54, "code": "PAK", "description": "Based on physical composition; Derived from analytical data; Known physical composition", "foodNutrientSource": { "id": 1, "code": "1", "description": "Analytical or derived from analytical" } }, "id": 1582487, "amount": 0.743, 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"unitName": "g" }, "foodNutrientDerivation": { "id": 54, "code": "PAK", "description": "Based on physical composition; Derived from analytical data; Known physical composition", "foodNutrientSource": { "id": 1, "code": "1", "description": "Analytical or derived from analytical" } }, "id": 1582517, "amount": 2.963, "dataPoints": 0 }, { "type": "FoodNutrient", "nutrient": { "id": 1215, "number": "506", "name": "Methionine", "rank": 16800, "unitName": "g" }, "foodNutrientDerivation": { "id": 54, "code": "PAK", "description": "Based on physical composition; Derived from analytical data; Known physical composition", "foodNutrientSource": { "id": 1, "code": "1", "description": "Analytical or derived from analytical" } }, "id": 1582488, "amount": 0.91, "dataPoints": 0 }, { "type": "FoodNutrient", "nutrient": { "id": 1216, "number": "507", "name": "Cystine", "rank": 16900, "unitName": "g" }, "foodNutrientDerivation": { "id": 54, "code": "PAK", "description": "Based on physical composition; 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"description": "Analytical or derived from analytical" } }, "id": 1582490, "amount": 1.005, "dataPoints": 0 }, { "type": "FoodNutrient", "nutrient": { "id": 1219, "number": "510", "name": "Valine", "rank": 17200, "unitName": "g" }, "foodNutrientDerivation": { "id": 54, "code": "PAK", "description": "Based on physical composition; Derived from analytical data; Known physical composition", "foodNutrientSource": { "id": 1, "code": "1", "description": "Analytical or derived from analytical" } }, "id": 1582519, "amount": 1.368, "dataPoints": 0 }, { "type": "FoodNutrient", "nutrient": { "id": 1220, "number": "511", "name": "Arginine", "rank": 17300, "unitName": "g" }, "foodNutrientDerivation": { "id": 54, "code": "PAK", "description": "Based on physical composition; Derived from analytical data; Known physical composition", "foodNutrientSource": { "id": 1, "code": "1", "description": "Analytical or derived from analytical" } }, "id": 1582520, "amount": 1.94, "dataPoints": 0 }, { "type": 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"amount": 1.0, "dataPoints": 0 }, { "type": "FoodNutrient", "nutrient": { "id": 1186, "number": "431", "name": "Folic acid", "rank": 7000, "unitName": "\u00b5g" }, "foodNutrientDerivation": { "id": 68, "code": "Z", "description": "Assumed zero (Insignificant amount or not naturally occurring in a food, such as fiber in meat)", "foodNutrientSource": { "id": 5, "code": "7", "description": "Assumed zero" } }, "id": 1687511, "amount": 0.0, "dataPoints": 0 }, { "type": "FoodNutrient", "nutrient": { "id": 1187, "number": "432", "name": "Folate, food", "rank": 7100, "unitName": "\u00b5g" }, "foodNutrientDerivation": { "id": 20, "code": "BFZN", "description": "Based on another form of the food or similar food; Concentration adjustment; No adjustment; Retention factors not used", "foodNutrientSource": { "id": 2, "code": "4", "description": "Calculated or imputed" } }, "id": 1687491, "amount": 1.0, "dataPoints": 0 }, { "type": "FoodNutrient", "nutrient": { "id": 1190, "number": "435", "name": 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J. Agric. Food Chem. 2003, 51, 14, 3940\u20133944. Normal Phase High-Performance Liquid Chromatography Method for the Determination of Tocopherols and Tocotrienols in Cereals", "loq": 19.9, "labMethodLink": "https://pubs.acs.org/doi/10.1021/jf030009v" }, { "subSampleId": 1755947, "nutrientId": 1129, "nutrientAcquisitionDetails": [ { "sampleUnitId": 1750601, "purchaseDate": "5/28/2020", "storeCity": "(Online Purchase)", "storeState": "MD", "packerCity": "Dallas", "packerState": "TX" } ], "amount": 0.0, "labMethodTechnique": "LC-FLD ()", "labMethodDescription": "AOAC 971.30 with HPLC quantification mod", "labMethodOriginalDescription": "Eurofins modified AOAC 971.30. J. Agric. Food Chem. 2003, 51, 14, 3940\u20133944. Normal Phase High-Performance Liquid Chromatography Method for the Determination of Tocopherols and Tocotrienols in Cereals", "loq": 20.0, "labMethodLink": "https://pubs.acs.org/doi/10.1021/jf030009v" }, { "subSampleId": 1756010, "nutrientId": 1129, "nutrientAcquisitionDetails": [ { "sampleUnitId": 1750603, "purchaseDate": "5/28/2020", "storeCity": "(Online Purchase)", "storeState": "MD", "packerCity": "Seattle", "packerState": "WA" } ], "amount": 0.0, "labMethodTechnique": "LC-FLD ()", "labMethodDescription": "AOAC 971.30 with HPLC quantification mod", "labMethodOriginalDescription": "Eurofins modified AOAC 971.30. J. Agric. Food Chem. 2003, 51, 14, 3940\u20133944. Normal Phase High-Performance Liquid Chromatography Method for the Determination of Tocopherols and Tocotrienols in Cereals", "loq": 19.7, "labMethodLink": "https://pubs.acs.org/doi/10.1021/jf030009v" }, { "subSampleId": 1756030, "nutrientId": 1129, "nutrientAcquisitionDetails": [ { "sampleUnitId": 1750605, "purchaseDate": "5/28/2020", "storeCity": "(Online Purchase)", "storeState": "MD", "packerCity": "Baltimore", "packerState": "MD" } ], "amount": 0.0, "labMethodTechnique": "LC-FLD ()", "labMethodDescription": "AOAC 971.30 with HPLC quantification mod", "labMethodOriginalDescription": "Eurofins modified AOAC 971.30. J. Agric. Food Chem. 2003, 51, 14, 3940\u20133944. Normal Phase High-Performance Liquid Chromatography Method for the Determination of Tocopherols and Tocotrienols in Cereals", "loq": 19.7, "labMethodLink": "https://pubs.acs.org/doi/10.1021/jf030009v" }, { "subSampleId": 1756126, "nutrientId": 1129, "nutrientAcquisitionDetails": [ { "sampleUnitId": 1750607, "purchaseDate": "5/29/2020", "storeCity": "(Online Purchase)", "storeState": "MD", "packerCity": "Dallas", "packerState": "TX" } ], "amount": 0.0, "labMethodTechnique": "LC-FLD ()", "labMethodDescription": "AOAC 971.30 with HPLC quantification mod", "labMethodOriginalDescription": "Eurofins modified AOAC 971.30. J. Agric. Food Chem. 2003, 51, 14, 3940\u20133944. 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Normal Phase High-Performance Liquid Chromatography Method for the Determination of Tocopherols and Tocotrienols in Cereals", "loq": 20.7, "labMethodLink": "https://pubs.acs.org/doi/10.1021/jf030009v" }, { "subSampleId": 1756269, "nutrientId": 1129, "nutrientAcquisitionDetails": [ { "sampleUnitId": 1750611, "purchaseDate": "6/1/2020", "storeCity": "(Online Purchase)", "storeState": "MD", "packerCity": "Bentonville", "packerState": "AR" } ], "amount": 0.0, "labMethodTechnique": "LC-FLD ()", "labMethodDescription": "AOAC 971.30 with HPLC quantification mod", "labMethodOriginalDescription": "Eurofins modified AOAC 971.30. J. Agric. Food Chem. 2003, 51, 14, 3940\u20133944. Normal Phase High-Performance Liquid Chromatography Method for the Determination of Tocopherols and Tocotrienols in Cereals", "loq": 20.6, "labMethodLink": "https://pubs.acs.org/doi/10.1021/jf030009v" }, { "subSampleId": 1756351, "nutrientId": 1129, "nutrientAcquisitionDetails": [ { "sampleUnitId": 1750613, "purchaseDate": "6/2/2020", "storeCity": "Columbia", "storeState": "MD", "packerCity": " Eden Prairie", "packerState": "MN" } ], "amount": 0.0, "labMethodTechnique": "LC-FLD ()", "labMethodDescription": "AOAC 971.30 with HPLC quantification mod", "labMethodOriginalDescription": "Eurofins modified AOAC 971.30. J. Agric. Food Chem. 2003, 51, 14, 3940\u20133944. Normal Phase High-Performance Liquid Chromatography Method for the Determination of Tocopherols and Tocotrienols in Cereals", "loq": 19.9, "labMethodLink": "https://pubs.acs.org/doi/10.1021/jf030009v" } ], "loq": 20.7 }, { "type": "FoodNutrient", "nutrient": { "id": 1130, "number": "346", "name": "Tocotrienol, gamma", "rank": 8500, "unitName": "mg" }, "foodNutrientDerivation": { "id": 1, "code": "A", "description": "Analytical", "foodNutrientSource": { "id": 1, "code": "1", "description": "Analytical or derived from analytical" } }, "id": 21114982, "amount": 0.0, "dataPoints": 8, "max": 0.0, "min": 0.0, "median": 0.0, "minYearAcquired": 2020, "nutrientAnalysisDetails": [ { "subSampleId": 1755805, "nutrientId": 1130, "nutrientAcquisitionDetails": [ { "sampleUnitId": 1750599, "purchaseDate": "5/28/2020", "storeCity": "(Online Purchase)", "storeState": "MD", "packerCity": "Lyndhurst", "packerState": "NJ" } ], "amount": 0.0, "labMethodTechnique": "LC-FLD ()", "labMethodDescription": "AOAC 971.30 with HPLC quantification mod", "labMethodOriginalDescription": "Eurofins modified AOAC 971.30. 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Normal Phase High-Performance Liquid Chromatography Method for the Determination of Tocopherols and Tocotrienols in Cereals", "loq": 20.0, "labMethodLink": "https://pubs.acs.org/doi/10.1021/jf030009v" }, { "subSampleId": 1756029, "nutrientId": 1130, "nutrientAcquisitionDetails": [ { "sampleUnitId": 1750603, "purchaseDate": "5/28/2020", "storeCity": "(Online Purchase)", "storeState": "MD", "packerCity": "Seattle", "packerState": "WA" } ], "amount": 0.0, "labMethodTechnique": "LC-FLD ()", "labMethodDescription": "AOAC 971.30 with HPLC quantification mod", "labMethodOriginalDescription": "Eurofins modified AOAC 971.30. J. Agric. Food Chem. 2003, 51, 14, 3940\u20133944. Normal Phase High-Performance Liquid Chromatography Method for the Determination of Tocopherols and Tocotrienols in Cereals", "loq": 19.7, "labMethodLink": "https://pubs.acs.org/doi/10.1021/jf030009v" }, { "subSampleId": 1756046, "nutrientId": 1130, "nutrientAcquisitionDetails": [ { "sampleUnitId": 1750605, "purchaseDate": "5/28/2020", "storeCity": "(Online Purchase)", "storeState": "MD", "packerCity": "Baltimore", "packerState": "MD" } ], "amount": 0.0, "labMethodTechnique": "LC-FLD ()", "labMethodDescription": "AOAC 971.30 with HPLC quantification mod", "labMethodOriginalDescription": "Eurofins modified AOAC 971.30. J. Agric. Food Chem. 2003, 51, 14, 3940\u20133944. Normal Phase High-Performance Liquid Chromatography Method for the Determination of Tocopherols and Tocotrienols in Cereals", "loq": 19.7, "labMethodLink": "https://pubs.acs.org/doi/10.1021/jf030009v" }, { "subSampleId": 1756128, "nutrientId": 1130, "nutrientAcquisitionDetails": [ { "sampleUnitId": 1750607, "purchaseDate": "5/29/2020", "storeCity": "(Online Purchase)", "storeState": "MD", "packerCity": "Dallas", "packerState": "TX" } ], "amount": 0.0, "labMethodTechnique": "LC-FLD ()", "labMethodDescription": "AOAC 971.30 with HPLC quantification mod", "labMethodOriginalDescription": "Eurofins modified AOAC 971.30. J. Agric. Food Chem. 2003, 51, 14, 3940\u20133944. Normal Phase High-Performance Liquid Chromatography Method for the Determination of Tocopherols and Tocotrienols in Cereals", "loq": 4.88, "labMethodLink": "https://pubs.acs.org/doi/10.1021/jf030009v" }, { "subSampleId": 1756258, "nutrientId": 1130, "nutrientAcquisitionDetails": [ { "sampleUnitId": 1750609, "purchaseDate": "5/29/2020", "storeCity": "(Online Purchase)", "storeState": "MD", "packerCity": "Minneapolis", "packerState": "MN" } ], "amount": 0.0, "labMethodTechnique": "LC-FLD ()", "labMethodDescription": "AOAC 971.30 with HPLC quantification mod", "labMethodOriginalDescription": "Eurofins modified AOAC 971.30. J. Agric. Food Chem. 2003, 51, 14, 3940\u20133944. Normal Phase High-Performance Liquid Chromatography Method for the Determination of Tocopherols and Tocotrienols in Cereals", "loq": 20.7, "labMethodLink": "https://pubs.acs.org/doi/10.1021/jf030009v" }, { "subSampleId": 1756283, "nutrientId": 1130, "nutrientAcquisitionDetails": [ { "sampleUnitId": 1750611, "purchaseDate": "6/1/2020", "storeCity": "(Online Purchase)", "storeState": "MD", "packerCity": "Bentonville", "packerState": "AR" } ], "amount": 0.0, "labMethodTechnique": "LC-FLD ()", "labMethodDescription": "AOAC 971.30 with HPLC quantification mod", "labMethodOriginalDescription": "Eurofins modified AOAC 971.30. J. Agric. Food Chem. 2003, 51, 14, 3940\u20133944. 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Normal Phase High-Performance Liquid Chromatography Method for the Determination of Tocopherols and Tocotrienols in Cereals", "loq": 19.7, "labMethodLink": "https://pubs.acs.org/doi/10.1021/jf030009v" }, { "subSampleId": 1756107, "nutrientId": 1131, "nutrientAcquisitionDetails": [ { "sampleUnitId": 1750607, "purchaseDate": "5/29/2020", "storeCity": "(Online Purchase)", "storeState": "MD", "packerCity": "Dallas", "packerState": "TX" } ], "amount": 0.0, "labMethodTechnique": "LC-FLD ()", "labMethodDescription": "AOAC 971.30 with HPLC quantification mod", "labMethodOriginalDescription": "Eurofins modified AOAC 971.30. J. Agric. Food Chem. 2003, 51, 14, 3940\u20133944. Normal Phase High-Performance Liquid Chromatography Method for the Determination of Tocopherols and Tocotrienols in Cereals", "loq": 4.88, "labMethodLink": "https://pubs.acs.org/doi/10.1021/jf030009v" }, { "subSampleId": 1756256, "nutrientId": 1131, "nutrientAcquisitionDetails": [ { "sampleUnitId": 1750609, "purchaseDate": "5/29/2020", "storeCity": "(Online Purchase)", "storeState": "MD", "packerCity": "Minneapolis", "packerState": "MN" } ], "amount": 0.0, "labMethodTechnique": "LC-FLD ()", "labMethodDescription": "AOAC 971.30 with HPLC quantification mod", "labMethodOriginalDescription": "Eurofins modified AOAC 971.30. J. Agric. Food Chem. 2003, 51, 14, 3940\u20133944. 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analytical" } }, "id": 24469959, "amount": 0.02167, "dataPoints": 6, "max": 0.13, "min": 0.0, "median": 0.0, "minYearAcquired": 2021, "nutrientAnalysisDetails": [ { "subSampleId": 2003486, "nutrientId": 1111, "nutrientAcquisitionDetails": [ { "sampleUnitId": 1999635, "purchaseDate": "1/5/2021", "storeCity": "(Region)", "storeState": "Atlantic", "packerCity": "", "packerState": "" } ], "amount": 0.0, "labMethodTechnique": "Direct alkaline saponification, SPE clean up, HPLC-UV", "labMethodDescription": "Publication forthcoming - validation data sent to MAFCL", "labMethodOriginalDescription": "Publication forthcoming - validation data sent to MAFCL", "labMethodLink": "https://www.aoac.org/official-methods-of-analysis-21st-edition-2019/" }, { "subSampleId": 2003487, "nutrientId": 1111, "nutrientAcquisitionDetails": [ { "sampleUnitId": 1999637, "purchaseDate": "1/5/2021", "storeCity": "(Region)", "storeState": "Atlantic", "packerCity": "", "packerState": "" } ], "amount": 0.0, "labMethodTechnique": "Direct alkaline saponification, SPE clean up, HPLC-UV", "labMethodDescription": "Publication forthcoming - validation data sent to MAFCL", "labMethodOriginalDescription": "Publication forthcoming - validation data sent to MAFCL", "labMethodLink": "https://www.aoac.org/official-methods-of-analysis-21st-edition-2019/" }, { "subSampleId": 2003488, "nutrientId": 1111, "nutrientAcquisitionDetails": [ { "sampleUnitId": 1999639, "purchaseDate": "1/11/2021", "storeCity": "(Region)", "storeState": "West", "packerCity": "", "packerState": "" } ], "amount": 0.0, "labMethodTechnique": "Direct alkaline saponification, SPE clean up, HPLC-UV", "labMethodDescription": "Publication forthcoming - validation data sent to MAFCL", "labMethodOriginalDescription": "Publication forthcoming - validation data sent to MAFCL", "labMethodLink": "https://www.aoac.org/official-methods-of-analysis-21st-edition-2019/" }, { "subSampleId": 2003489, "nutrientId": 1111, "nutrientAcquisitionDetails": [ { "sampleUnitId": 1999641, "purchaseDate": "1/5/2021", "storeCity": "(Region)", "storeState": "Plains", "packerCity": "", "packerState": "" } ], "amount": 0.0, "labMethodTechnique": "Direct alkaline saponification, SPE clean up, HPLC-UV", "labMethodDescription": "Publication forthcoming - validation data sent to MAFCL", "labMethodOriginalDescription": "Publication forthcoming - validation data sent to MAFCL", "labMethodLink": "https://www.aoac.org/official-methods-of-analysis-21st-edition-2019/" }, { "subSampleId": 2003490, "nutrientId": 1111, "nutrientAcquisitionDetails": [ { "sampleUnitId": 1999643, "purchaseDate": "1/5/2021", "storeCity": "(Region)", "storeState": "Plains", "packerCity": "", "packerState": "" } ], "amount": 0.13, "labMethodTechnique": "Direct alkaline saponification, SPE clean up, HPLC-UV", "labMethodDescription": "Publication forthcoming - validation data sent to MAFCL", "labMethodOriginalDescription": "Publication forthcoming - validation data sent to MAFCL", "labMethodLink": "https://www.aoac.org/official-methods-of-analysis-21st-edition-2019/" }, { "subSampleId": 2003491, "nutrientId": 1111, "nutrientAcquisitionDetails": [ { "sampleUnitId": 1999645, "purchaseDate": "1/19/2021", "storeCity": "Blacksburg", "storeState": "VA", "packerCity": "", "packerState": "" } ], "amount": 0.0, "labMethodTechnique": "Direct alkaline saponification, SPE clean up, HPLC-UV", "labMethodDescription": "Publication forthcoming - validation data sent to MAFCL", "labMethodOriginalDescription": "Publication forthcoming - validation data sent to MAFCL", "labMethodLink": "https://www.aoac.org/official-methods-of-analysis-21st-edition-2019/" } ] }, { "type": "FoodNutrient", "nutrient": { "id": 2059, "number": "", "name": "Vitamin D4", "rank": 8730, "unitName": "\u00b5g" }, "foodNutrientDerivation": { "id": 1, "code": "A", "description": "Analytical", "foodNutrientSource": { "id": 1, "code": "1", "description": "Analytical or derived from analytical" } }, "id": 24469947, "amount": 0.0, "dataPoints": 6, "max": 0.0, "min": 0.0, "median": 0.0, "minYearAcquired": 2021, "nutrientAnalysisDetails": [ { "subSampleId": 2003216, "nutrientId": 2059, "nutrientAcquisitionDetails": [ { "sampleUnitId": 1999635, "purchaseDate": "1/5/2021", "storeCity": "(Region)", "storeState": "Atlantic", "packerCity": "", "packerState": "" } ], "amount": 0.0, "labMethodTechnique": "Direct alkaline saponification, SPE clean up, HPLC-UV", "labMethodDescription": "Publication forthcoming - validation data sent to MAFCL", "labMethodOriginalDescription": "Publication forthcoming - validation data sent to MAFCL", "labMethodLink": "https://www.aoac.org/official-methods-of-analysis-21st-edition-2019/" }, { "subSampleId": 2003217, "nutrientId": 2059, "nutrientAcquisitionDetails": [ { "sampleUnitId": 1999637, "purchaseDate": "1/5/2021", "storeCity": "(Region)", "storeState": "Atlantic", "packerCity": "", "packerState": "" } ], "amount": 0.0, "labMethodTechnique": "Direct alkaline saponification, SPE clean up, HPLC-UV", "labMethodDescription": "Publication forthcoming - validation data sent to MAFCL", "labMethodOriginalDescription": "Publication forthcoming - validation data sent to MAFCL", "labMethodLink": "https://www.aoac.org/official-methods-of-analysis-21st-edition-2019/" }, { "subSampleId": 2003218, "nutrientId": 2059, "nutrientAcquisitionDetails": [ { "sampleUnitId": 1999639, "purchaseDate": "1/11/2021", "storeCity": "(Region)", "storeState": "West", "packerCity": "", "packerState": "" } ], "amount": 0.0, "labMethodTechnique": "Direct alkaline saponification, SPE clean up, HPLC-UV", "labMethodDescription": "Publication forthcoming - validation data sent to MAFCL", "labMethodOriginalDescription": "Publication forthcoming - validation data sent to MAFCL", "labMethodLink": "https://www.aoac.org/official-methods-of-analysis-21st-edition-2019/" }, { "subSampleId": 2003219, "nutrientId": 2059, "nutrientAcquisitionDetails": [ { "sampleUnitId": 1999641, "purchaseDate": "1/5/2021", "storeCity": "(Region)", "storeState": "Plains", "packerCity": "", "packerState": "" } ], "amount": 0.0, "labMethodTechnique": "Direct alkaline saponification, SPE clean up, HPLC-UV", "labMethodDescription": "Publication forthcoming - validation data sent to MAFCL", "labMethodOriginalDescription": "Publication forthcoming - validation data sent to MAFCL", "labMethodLink": "https://www.aoac.org/official-methods-of-analysis-21st-edition-2019/" }, { "subSampleId": 2003220, "nutrientId": 2059, "nutrientAcquisitionDetails": [ { "sampleUnitId": 1999643, "purchaseDate": "1/5/2021", "storeCity": "(Region)", "storeState": "Plains", "packerCity": "", "packerState": "" } ], "amount": 0.0, "labMethodTechnique": "Direct alkaline saponification, SPE clean up, HPLC-UV", "labMethodDescription": "Publication forthcoming - validation data sent to MAFCL", "labMethodOriginalDescription": "Publication forthcoming - validation data sent to MAFCL", "labMethodLink": "https://www.aoac.org/official-methods-of-analysis-21st-edition-2019/" }, { "subSampleId": 2003221, "nutrientId": 2059, "nutrientAcquisitionDetails": [ { "sampleUnitId": 1999645, "purchaseDate": "1/19/2021", "storeCity": "Blacksburg", "storeState": "VA", "packerCity": "", "packerState": "" } ], "amount": 0.0, "labMethodTechnique": "Direct alkaline saponification, SPE clean up, HPLC-UV", "labMethodDescription": "Publication forthcoming - validation data sent to MAFCL", "labMethodOriginalDescription": "Publication forthcoming - validation data sent to MAFCL", "labMethodLink": "https://www.aoac.org/official-methods-of-analysis-21st-edition-2019/" } ] }, { "type": "FoodNutrient", "nutrient": { "id": 2069, "number": "961", "name": "Glutathione", "rank": 9000, "unitName": "mg" }, "foodNutrientDerivation": { "id": 1, "code": "A", "description": "Analytical", "foodNutrientSource": { "id": 1, "code": "1", "description": "Analytical or derived from analytical" } }, "id": 33291524, "amount": 4.3, "dataPoints": 6, "max": 7.2, "min": 2.9, "median": 3.68, "minYearAcquired": 2021, "nutrientAnalysisDetails": [ { "subSampleId": 2643716, "nutrientId": 2069, "nutrientAcquisitionDetails": [ { "sampleUnitId": 1999635, "purchaseDate": "1/5/2021", "storeCity": "(Region)", "storeState": "Atlantic", "packerCity": "", "packerState": "" } ], "amount": 3.4, "labMethodTechnique": "LC-QqQ MS", "labMethodDescription": "Ethanol extraction along with dithiothreitol (DTT) reduction, followed by LC-MS/MS analysis", "labMethodOriginalDescription": "One gram of undried mushroom samples was weighed in 15 mL polypropylene tubes and kept on ice. To inhibit endogenous enzyme activities, 4 mL of ice-cold ethanol and 550 \u03bcL of a mixture of a protease inhibitor cocktail (ReadyShield\u00ae PIC0005) and 50 mM EDTA disodium salt in 100 mM ammonium bicarbonate buffer, pH 8.5 (1:10; v/v) was added to the mushroom samples. Samples were mixed by vortex. An internal standard of 200 \u03bcL of 100 \u03bcg/mL\u00a0glutathione-(glycine-13C2, 15N) was added to the tubes, followed by adding 350 \u03bcL of 265 mg/mL dithiothreitol (DTT) prepared\u00a0in 100 mM ammonium bicarbonate buffer, pH 8.5. After mixing samples by vortex, samples were incubated at 37\u00b0C for 2 h with continuous vigorous shaking. Two milliliters of ethanol were added to the samples, which were then mixed by vortex and centrifuged at 4,000 g at 4\u00b0C for 15 min. The supernatants were decanted into new 15 mL polypropylene tubes. The pellets were suspended in 7 mL of 75% ethanol by mixing with glass stirring rods and vortex. Samples were centrifuged with the above condition. The two supernatants were combined and mixed by inverting the tubes several times. An aliquot of 150 \u03bcL of combined supernatants was transferred to 1.5 mL microcentrifuge\u00a0tubes and dried by speedvac. The dried samples were dissolved in 100 \u03bcL of pure water and then filtered with 0.2 \u03bcm polyethersulfone filter plates. \nGlutathione concentration was determined by liquid chromatography triple quadrupole mass spectrometer (Agilent 1260 Infinity II LC system with 6470 Triple Quadrupole LC-MS) using multiple reaction monitoring (MRM) analysis. Chromatographic separation was performed on an Agilent AdvanceBio Peptide Map (2.1\u00a0\u00d7 150 mm, 2.7 \u03bcm) analytical column with an AdvanceBio Peptide Map guard column (2.1 \u00d7 5 mm, 2.7 \u03bcm) at a flow rate of 0.3 mL/min. The mobile phase gradient composed of 0.1 % formic acid and 3% acetonitrile\u00a0in water (v/v/v) (solvent A) and 0.1 % formic acid and 10% water in acetonitrile (v/v/v) (solvent B) was as follows: 0-16% B from 0-2 min; 16-100% B from 2-3 min; 100% B from 3-4.5 min; 100-0% B 4.5-5 min. The column was equilibrated with 100% A for 8 min before the next injection. MRM analysis was conducted in the positive mode with one transition as the quantifier and two transitions as qualifiers for the analyte and the internal standard. For glutathione, the quantifier was m/z 308.0 -> 179.0, and the qualifiers were m/z\u00a0308.0 -> 233.0 and m/z 308.0 -> 162.0. For glutathione-(glycine-13C2, 15N), the quantifier was m/z 311.0 -> 182.0, and the qualifiers were m/z\u00a0311.0 -> 165.0 and m/z 311.0 -> 84.0. Calibration standards of glutathione with a concentration range of 0.1-50 \u03bcg/mL and 2 \u03bcg/mL of internal standard were prepared in 20 mM DTT.", "labMethodLink": "https://www.aoac.org/official-methods-of-analysis-21st-edition-2019/" }, { "subSampleId": 2643717, "nutrientId": 2069, "nutrientAcquisitionDetails": [ { "sampleUnitId": 1999637, "purchaseDate": "1/5/2021", "storeCity": "(Region)", "storeState": "Atlantic", "packerCity": "", "packerState": "" } ], "amount": 7.2, "labMethodTechnique": "LC-QqQ MS", "labMethodDescription": "Ethanol extraction along with dithiothreitol (DTT) reduction, followed by LC-MS/MS analysis", "labMethodOriginalDescription": "One gram of undried mushroom samples was weighed in 15 mL polypropylene tubes and kept on ice. To inhibit endogenous enzyme activities, 4 mL of ice-cold ethanol and 550 \u03bcL of a mixture of a protease inhibitor cocktail (ReadyShield\u00ae PIC0005) and 50 mM EDTA disodium salt in 100 mM ammonium bicarbonate buffer, pH 8.5 (1:10; v/v) was added to the mushroom samples. Samples were mixed by vortex. An internal standard of 200 \u03bcL of 100 \u03bcg/mL\u00a0glutathione-(glycine-13C2, 15N) was added to the tubes, followed by adding 350 \u03bcL of 265 mg/mL dithiothreitol (DTT) prepared\u00a0in 100 mM ammonium bicarbonate buffer, pH 8.5. After mixing samples by vortex, samples were incubated at 37\u00b0C for 2 h with continuous vigorous shaking. Two milliliters of ethanol were added to the samples, which were then mixed by vortex and centrifuged at 4,000 g at 4\u00b0C for 15 min. The supernatants were decanted into new 15 mL polypropylene tubes. The pellets were suspended in 7 mL of 75% ethanol by mixing with glass stirring rods and vortex. Samples were centrifuged with the above condition. The two supernatants were combined and mixed by inverting the tubes several times. An aliquot of 150 \u03bcL of combined supernatants was transferred to 1.5 mL microcentrifuge\u00a0tubes and dried by speedvac. The dried samples were dissolved in 100 \u03bcL of pure water and then filtered with 0.2 \u03bcm polyethersulfone filter plates. \nGlutathione concentration was determined by liquid chromatography triple quadrupole mass spectrometer (Agilent 1260 Infinity II LC system with 6470 Triple Quadrupole LC-MS) using multiple reaction monitoring (MRM) analysis. Chromatographic separation was performed on an Agilent AdvanceBio Peptide Map (2.1\u00a0\u00d7 150 mm, 2.7 \u03bcm) analytical column with an AdvanceBio Peptide Map guard column (2.1 \u00d7 5 mm, 2.7 \u03bcm) at a flow rate of 0.3 mL/min. The mobile phase gradient composed of 0.1 % formic acid and 3% acetonitrile\u00a0in water (v/v/v) (solvent A) and 0.1 % formic acid and 10% water in acetonitrile (v/v/v) (solvent B) was as follows: 0-16% B from 0-2 min; 16-100% B from 2-3 min; 100% B from 3-4.5 min; 100-0% B 4.5-5 min. The column was equilibrated with 100% A for 8 min before the next injection. MRM analysis was conducted in the positive mode with one transition as the quantifier and two transitions as qualifiers for the analyte and the internal standard. For glutathione, the quantifier was m/z 308.0 -> 179.0, and the qualifiers were m/z\u00a0308.0 -> 233.0 and m/z 308.0 -> 162.0. For glutathione-(glycine-13C2, 15N), the quantifier was m/z 311.0 -> 182.0, and the qualifiers were m/z\u00a0311.0 -> 165.0 and m/z 311.0 -> 84.0. Calibration standards of glutathione with a concentration range of 0.1-50 \u03bcg/mL and 2 \u03bcg/mL of internal standard were prepared in 20 mM DTT.", "labMethodLink": "https://www.aoac.org/official-methods-of-analysis-21st-edition-2019/" }, { "subSampleId": 2643718, "nutrientId": 2069, "nutrientAcquisitionDetails": [ { "sampleUnitId": 1999639, "purchaseDate": "1/11/2021", "storeCity": "(Region)", "storeState": "West", "packerCity": "", "packerState": "" } ], "amount": 4, "labMethodTechnique": "LC-QqQ MS", "labMethodDescription": "Ethanol extraction along with dithiothreitol (DTT) reduction, followed by LC-MS/MS analysis", "labMethodOriginalDescription": "One gram of undried mushroom samples was weighed in 15 mL polypropylene tubes and kept on ice. To inhibit endogenous enzyme activities, 4 mL of ice-cold ethanol and 550 \u03bcL of a mixture of a protease inhibitor cocktail (ReadyShield\u00ae PIC0005) and 50 mM EDTA disodium salt in 100 mM ammonium bicarbonate buffer, pH 8.5 (1:10; v/v) was added to the mushroom samples. Samples were mixed by vortex. An internal standard of 200 \u03bcL of 100 \u03bcg/mL\u00a0glutathione-(glycine-13C2, 15N) was added to the tubes, followed by adding 350 \u03bcL of 265 mg/mL dithiothreitol (DTT) prepared\u00a0in 100 mM ammonium bicarbonate buffer, pH 8.5. After mixing samples by vortex, samples were incubated at 37\u00b0C for 2 h with continuous vigorous shaking. Two milliliters of ethanol were added to the samples, which were then mixed by vortex and centrifuged at 4,000 g at 4\u00b0C for 15 min. The supernatants were decanted into new 15 mL polypropylene tubes. The pellets were suspended in 7 mL of 75% ethanol by mixing with glass stirring rods and vortex. Samples were centrifuged with the above condition. The two supernatants were combined and mixed by inverting the tubes several times. An aliquot of 150 \u03bcL of combined supernatants was transferred to 1.5 mL microcentrifuge\u00a0tubes and dried by speedvac. The dried samples were dissolved in 100 \u03bcL of pure water and then filtered with 0.2 \u03bcm polyethersulfone filter plates. \nGlutathione concentration was determined by liquid chromatography triple quadrupole mass spectrometer (Agilent 1260 Infinity II LC system with 6470 Triple Quadrupole LC-MS) using multiple reaction monitoring (MRM) analysis. Chromatographic separation was performed on an Agilent AdvanceBio Peptide Map (2.1\u00a0\u00d7 150 mm, 2.7 \u03bcm) analytical column with an AdvanceBio Peptide Map guard column (2.1 \u00d7 5 mm, 2.7 \u03bcm) at a flow rate of 0.3 mL/min. The mobile phase gradient composed of 0.1 % formic acid and 3% acetonitrile\u00a0in water (v/v/v) (solvent A) and 0.1 % formic acid and 10% water in acetonitrile (v/v/v) (solvent B) was as follows: 0-16% B from 0-2 min; 16-100% B from 2-3 min; 100% B from 3-4.5 min; 100-0% B 4.5-5 min. The column was equilibrated with 100% A for 8 min before the next injection. MRM analysis was conducted in the positive mode with one transition as the quantifier and two transitions as qualifiers for the analyte and the internal standard. For glutathione, the quantifier was m/z 308.0 -> 179.0, and the qualifiers were m/z\u00a0308.0 -> 233.0 and m/z 308.0 -> 162.0. For glutathione-(glycine-13C2, 15N), the quantifier was m/z 311.0 -> 182.0, and the qualifiers were m/z\u00a0311.0 -> 165.0 and m/z 311.0 -> 84.0. Calibration standards of glutathione with a concentration range of 0.1-50 \u03bcg/mL and 2 \u03bcg/mL of internal standard were prepared in 20 mM DTT.", "labMethodLink": "https://www.aoac.org/official-methods-of-analysis-21st-edition-2019/" }, { "subSampleId": 2643719, "nutrientId": 2069, "nutrientAcquisitionDetails": [ { "sampleUnitId": 1999641, "purchaseDate": "1/5/2021", "storeCity": "(Region)", "storeState": "Plains", "packerCity": "", "packerState": "" } ], "amount": 2.9, "labMethodTechnique": "LC-QqQ MS", "labMethodDescription": "Ethanol extraction along with dithiothreitol (DTT) reduction, followed by LC-MS/MS analysis", "labMethodOriginalDescription": "One gram of undried mushroom samples was weighed in 15 mL polypropylene tubes and kept on ice. To inhibit endogenous enzyme activities, 4 mL of ice-cold ethanol and 550 \u03bcL of a mixture of a protease inhibitor cocktail (ReadyShield\u00ae PIC0005) and 50 mM EDTA disodium salt in 100 mM ammonium bicarbonate buffer, pH 8.5 (1:10; v/v) was added to the mushroom samples. Samples were mixed by vortex. An internal standard of 200 \u03bcL of 100 \u03bcg/mL\u00a0glutathione-(glycine-13C2, 15N) was added to the tubes, followed by adding 350 \u03bcL of 265 mg/mL dithiothreitol (DTT) prepared\u00a0in 100 mM ammonium bicarbonate buffer, pH 8.5. After mixing samples by vortex, samples were incubated at 37\u00b0C for 2 h with continuous vigorous shaking. Two milliliters of ethanol were added to the samples, which were then mixed by vortex and centrifuged at 4,000 g at 4\u00b0C for 15 min. The supernatants were decanted into new 15 mL polypropylene tubes. The pellets were suspended in 7 mL of 75% ethanol by mixing with glass stirring rods and vortex. Samples were centrifuged with the above condition. The two supernatants were combined and mixed by inverting the tubes several times. An aliquot of 150 \u03bcL of combined supernatants was transferred to 1.5 mL microcentrifuge\u00a0tubes and dried by speedvac. The dried samples were dissolved in 100 \u03bcL of pure water and then filtered with 0.2 \u03bcm polyethersulfone filter plates. \nGlutathione concentration was determined by liquid chromatography triple quadrupole mass spectrometer (Agilent 1260 Infinity II LC system with 6470 Triple Quadrupole LC-MS) using multiple reaction monitoring (MRM) analysis. Chromatographic separation was performed on an Agilent AdvanceBio Peptide Map (2.1\u00a0\u00d7 150 mm, 2.7 \u03bcm) analytical column with an AdvanceBio Peptide Map guard column (2.1 \u00d7 5 mm, 2.7 \u03bcm) at a flow rate of 0.3 mL/min. The mobile phase gradient composed of 0.1 % formic acid and 3% acetonitrile\u00a0in water (v/v/v) (solvent A) and 0.1 % formic acid and 10% water in acetonitrile (v/v/v) (solvent B) was as follows: 0-16% B from 0-2 min; 16-100% B from 2-3 min; 100% B from 3-4.5 min; 100-0% B 4.5-5 min. The column was equilibrated with 100% A for 8 min before the next injection. MRM analysis was conducted in the positive mode with one transition as the quantifier and two transitions as qualifiers for the analyte and the internal standard. For glutathione, the quantifier was m/z 308.0 -> 179.0, and the qualifiers were m/z\u00a0308.0 -> 233.0 and m/z 308.0 -> 162.0. For glutathione-(glycine-13C2, 15N), the quantifier was m/z 311.0 -> 182.0, and the qualifiers were m/z\u00a0311.0 -> 165.0 and m/z 311.0 -> 84.0. Calibration standards of glutathione with a concentration range of 0.1-50 \u03bcg/mL and 2 \u03bcg/mL of internal standard were prepared in 20 mM DTT.", "labMethodLink": "https://www.aoac.org/official-methods-of-analysis-21st-edition-2019/" }, { "subSampleId": 2643720, "nutrientId": 2069, "nutrientAcquisitionDetails": [ { "sampleUnitId": 1999643, "purchaseDate": "1/5/2021", "storeCity": "(Region)", "storeState": "Plains", "packerCity": "", "packerState": "" } ], "amount": 5, "labMethodTechnique": "LC-QqQ MS", "labMethodDescription": "Ethanol extraction along with dithiothreitol (DTT) reduction, followed by LC-MS/MS analysis", "labMethodOriginalDescription": "One gram of undried mushroom samples was weighed in 15 mL polypropylene tubes and kept on ice. To inhibit endogenous enzyme activities, 4 mL of ice-cold ethanol and 550 \u03bcL of a mixture of a protease inhibitor cocktail (ReadyShield\u00ae PIC0005) and 50 mM EDTA disodium salt in 100 mM ammonium bicarbonate buffer, pH 8.5 (1:10; v/v) was added to the mushroom samples. Samples were mixed by vortex. An internal standard of 200 \u03bcL of 100 \u03bcg/mL\u00a0glutathione-(glycine-13C2, 15N) was added to the tubes, followed by adding 350 \u03bcL of 265 mg/mL dithiothreitol (DTT) prepared\u00a0in 100 mM ammonium bicarbonate buffer, pH 8.5. After mixing samples by vortex, samples were incubated at 37\u00b0C for 2 h with continuous vigorous shaking. Two milliliters of ethanol were added to the samples, which were then mixed by vortex and centrifuged at 4,000 g at 4\u00b0C for 15 min. The supernatants were decanted into new 15 mL polypropylene tubes. The pellets were suspended in 7 mL of 75% ethanol by mixing with glass stirring rods and vortex. Samples were centrifuged with the above condition. The two supernatants were combined and mixed by inverting the tubes several times. An aliquot of 150 \u03bcL of combined supernatants was transferred to 1.5 mL microcentrifuge\u00a0tubes and dried by speedvac. The dried samples were dissolved in 100 \u03bcL of pure water and then filtered with 0.2 \u03bcm polyethersulfone filter plates. \nGlutathione concentration was determined by liquid chromatography triple quadrupole mass spectrometer (Agilent 1260 Infinity II LC system with 6470 Triple Quadrupole LC-MS) using multiple reaction monitoring (MRM) analysis. Chromatographic separation was performed on an Agilent AdvanceBio Peptide Map (2.1\u00a0\u00d7 150 mm, 2.7 \u03bcm) analytical column with an AdvanceBio Peptide Map guard column (2.1 \u00d7 5 mm, 2.7 \u03bcm) at a flow rate of 0.3 mL/min. The mobile phase gradient composed of 0.1 % formic acid and 3% acetonitrile\u00a0in water (v/v/v) (solvent A) and 0.1 % formic acid and 10% water in acetonitrile (v/v/v) (solvent B) was as follows: 0-16% B from 0-2 min; 16-100% B from 2-3 min; 100% B from 3-4.5 min; 100-0% B 4.5-5 min. The column was equilibrated with 100% A for 8 min before the next injection. MRM analysis was conducted in the positive mode with one transition as the quantifier and two transitions as qualifiers for the analyte and the internal standard. For glutathione, the quantifier was m/z 308.0 -> 179.0, and the qualifiers were m/z\u00a0308.0 -> 233.0 and m/z 308.0 -> 162.0. For glutathione-(glycine-13C2, 15N), the quantifier was m/z 311.0 -> 182.0, and the qualifiers were m/z\u00a0311.0 -> 165.0 and m/z 311.0 -> 84.0. Calibration standards of glutathione with a concentration range of 0.1-50 \u03bcg/mL and 2 \u03bcg/mL of internal standard were prepared in 20 mM DTT.", "labMethodLink": "https://www.aoac.org/official-methods-of-analysis-21st-edition-2019/" }, { "subSampleId": 2643721, "nutrientId": 2069, "nutrientAcquisitionDetails": [ { "sampleUnitId": 1999645, "purchaseDate": "1/19/2021", "storeCity": "Blacksburg", "storeState": "VA", "packerCity": "", "packerState": "" } ], "amount": 3.1, "labMethodTechnique": "LC-QqQ MS", "labMethodDescription": "Ethanol extraction along with dithiothreitol (DTT) reduction, followed by LC-MS/MS analysis", "labMethodOriginalDescription": "One gram of undried mushroom samples was weighed in 15 mL polypropylene tubes and kept on ice. To inhibit endogenous enzyme activities, 4 mL of ice-cold ethanol and 550 \u03bcL of a mixture of a protease inhibitor cocktail (ReadyShield\u00ae PIC0005) and 50 mM EDTA disodium salt in 100 mM ammonium bicarbonate buffer, pH 8.5 (1:10; v/v) was added to the mushroom samples. Samples were mixed by vortex. An internal standard of 200 \u03bcL of 100 \u03bcg/mL\u00a0glutathione-(glycine-13C2, 15N) was added to the tubes, followed by adding 350 \u03bcL of 265 mg/mL dithiothreitol (DTT) prepared\u00a0in 100 mM ammonium bicarbonate buffer, pH 8.5. After mixing samples by vortex, samples were incubated at 37\u00b0C for 2 h with continuous vigorous shaking. Two milliliters of ethanol were added to the samples, which were then mixed by vortex and centrifuged at 4,000 g at 4\u00b0C for 15 min. The supernatants were decanted into new 15 mL polypropylene tubes. The pellets were suspended in 7 mL of 75% ethanol by mixing with glass stirring rods and vortex. Samples were centrifuged with the above condition. The two supernatants were combined and mixed by inverting the tubes several times. An aliquot of 150 \u03bcL of combined supernatants was transferred to 1.5 mL microcentrifuge\u00a0tubes and dried by speedvac. The dried samples were dissolved in 100 \u03bcL of pure water and then filtered with 0.2 \u03bcm polyethersulfone filter plates. \nGlutathione concentration was determined by liquid chromatography triple quadrupole mass spectrometer (Agilent 1260 Infinity II LC system with 6470 Triple Quadrupole LC-MS) using multiple reaction monitoring (MRM) analysis. Chromatographic separation was performed on an Agilent AdvanceBio Peptide Map (2.1\u00a0\u00d7 150 mm, 2.7 \u03bcm) analytical column with an AdvanceBio Peptide Map guard column (2.1 \u00d7 5 mm, 2.7 \u03bcm) at a flow rate of 0.3 mL/min. The mobile phase gradient composed of 0.1 % formic acid and 3% acetonitrile\u00a0in water (v/v/v) (solvent A) and 0.1 % formic acid and 10% water in acetonitrile (v/v/v) (solvent B) was as follows: 0-16% B from 0-2 min; 16-100% B from 2-3 min; 100% B from 3-4.5 min; 100-0% B 4.5-5 min. The column was equilibrated with 100% A for 8 min before the next injection. MRM analysis was conducted in the positive mode with one transition as the quantifier and two transitions as qualifiers for the analyte and the internal standard. For glutathione, the quantifier was m/z 308.0 -> 179.0, and the qualifiers were m/z\u00a0308.0 -> 233.0 and m/z 308.0 -> 162.0. For glutathione-(glycine-13C2, 15N), the quantifier was m/z 311.0 -> 182.0, and the qualifiers were m/z\u00a0311.0 -> 165.0 and m/z 311.0 -> 84.0. Calibration standards of glutathione with a concentration range of 0.1-50 \u03bcg/mL and 2 \u03bcg/mL of internal standard were prepared in 20 mM DTT.", "labMethodLink": "https://www.aoac.org/official-methods-of-analysis-21st-edition-2019/" } ] }, { "nutrient": { "id": 1283, "number": "636", "name": "Phytosterols", "rank": 15800, "unitName": "mg" }, "type": "FoodNutrient" }, { "type": "FoodNutrient", "nutrient": { "id": 1285, "number": "638", "name": "Stigmasterol", "rank": 15900, "unitName": "mg" }, "foodNutrientDerivation": { "id": 1, "code": "A", "description": "Analytical", "foodNutrientSource": { "id": 1, "code": "1", "description": "Analytical or derived from analytical" } }, "id": 24469941, "amount": 0.0, "dataPoints": 6, "max": 0.0, "min": 0.0, "median": 0.0, "minYearAcquired": 2021, "nutrientAnalysisDetails": [ { "subSampleId": 2003396, "nutrientId": 1285, "nutrientAcquisitionDetails": [ { "sampleUnitId": 1999635, "purchaseDate": "1/5/2021", "storeCity": "(Region)", "storeState": "Atlantic", "packerCity": "", "packerState": "" } ], "amount": 0.0, "labMethodTechnique": "Direct alkaline saponification, BSTFA/TMCS derivatization; GC-FID", "labMethodDescription": "Phyto- & Fungisterols", "labMethodOriginalDescription": "Phyto- & Fungisterols (direct saponification, free and esterified)", "loq": 0.2, "labMethodLink": "" }, { "subSampleId": 2003397, "nutrientId": 1285, "nutrientAcquisitionDetails": [ { "sampleUnitId": 1999637, "purchaseDate": "1/5/2021", "storeCity": "(Region)", "storeState": "Atlantic", "packerCity": "", "packerState": "" } ], "amount": 0.0, "labMethodTechnique": "Direct alkaline saponification, BSTFA/TMCS derivatization; GC-FID", "labMethodDescription": "Phyto- & Fungisterols", "labMethodOriginalDescription": "Phyto- & Fungisterols (direct saponification, free and esterified)", "loq": 0.2, "labMethodLink": "" }, { "subSampleId": 2003398, "nutrientId": 1285, "nutrientAcquisitionDetails": [ { "sampleUnitId": 1999639, "purchaseDate": "1/11/2021", "storeCity": "(Region)", "storeState": "West", "packerCity": "", "packerState": "" } ], "amount": 0.0, "labMethodTechnique": "Direct alkaline saponification, BSTFA/TMCS derivatization; GC-FID", "labMethodDescription": "Phyto- & Fungisterols", "labMethodOriginalDescription": "Phyto- & Fungisterols (direct saponification, free and esterified)", "loq": 0.2, "labMethodLink": "" }, { "subSampleId": 2003399, "nutrientId": 1285, "nutrientAcquisitionDetails": [ { "sampleUnitId": 1999641, "purchaseDate": "1/5/2021", "storeCity": "(Region)", "storeState": "Plains", "packerCity": "", "packerState": "" } ], "amount": 0.0, "labMethodTechnique": "Direct alkaline saponification, BSTFA/TMCS derivatization; GC-FID", "labMethodDescription": "Phyto- & Fungisterols", "labMethodOriginalDescription": "Phyto- & Fungisterols (direct saponification, free and esterified)", "loq": 0.2, "labMethodLink": "" }, { "subSampleId": 2003400, "nutrientId": 1285, "nutrientAcquisitionDetails": [ { "sampleUnitId": 1999643, "purchaseDate": "1/5/2021", "storeCity": "(Region)", "storeState": "Plains", "packerCity": "", "packerState": "" } ], "amount": 0.0, "labMethodTechnique": "Direct alkaline saponification, BSTFA/TMCS derivatization; GC-FID", "labMethodDescription": "Phyto- & Fungisterols", "labMethodOriginalDescription": "Phyto- & Fungisterols (direct saponification, free and esterified)", "loq": 0.2, "labMethodLink": "" }, { "subSampleId": 2003401, "nutrientId": 1285, "nutrientAcquisitionDetails": [ { "sampleUnitId": 1999645, "purchaseDate": "1/19/2021", "storeCity": "Blacksburg", "storeState": "VA", "packerCity": "", "packerState": "" } ], "amount": 0.0, "labMethodTechnique": "Direct alkaline saponification, BSTFA/TMCS derivatization; GC-FID", "labMethodDescription": "Phyto- & Fungisterols", "labMethodOriginalDescription": "Phyto- & Fungisterols (direct saponification, free and esterified)", "loq": 0.2, "labMethodLink": "" } ], "loq": 0.2 }, { "type": "FoodNutrient", "nutrient": { "id": 1286, "number": "639", "name": "Campesterol", "rank": 16000, "unitName": "mg" }, "foodNutrientDerivation": { "id": 1, "code": "A", "description": "Analytical", "foodNutrientSource": { "id": 1, "code": "1", "description": "Analytical or derived from analytical" } }, "id": 24469942, "amount": 0.0, "dataPoints": 6, "max": 0.0, "min": 0.0, "median": 0.0, "minYearAcquired": 2021, "nutrientAnalysisDetails": [ { "subSampleId": 2003366, "nutrientId": 1286, "nutrientAcquisitionDetails": [ { "sampleUnitId": 1999635, "purchaseDate": "1/5/2021", "storeCity": "(Region)", "storeState": "Atlantic", "packerCity": "", "packerState": "" } ], "amount": 0.0, "labMethodTechnique": "Direct alkaline saponification, BSTFA/TMCS derivatization; GC-FID", "labMethodDescription": "Phyto- & Fungisterols", "labMethodOriginalDescription": "Phyto- & Fungisterols (direct saponification, free and esterified)", "loq": 0.2, "labMethodLink": "" }, { "subSampleId": 2003367, "nutrientId": 1286, "nutrientAcquisitionDetails": [ { "sampleUnitId": 1999637, "purchaseDate": "1/5/2021", "storeCity": "(Region)", "storeState": "Atlantic", "packerCity": "", "packerState": "" } ], "amount": 0.0, "labMethodTechnique": "Direct alkaline saponification, BSTFA/TMCS derivatization; GC-FID", "labMethodDescription": "Phyto- & Fungisterols", "labMethodOriginalDescription": "Phyto- & Fungisterols (direct saponification, free and esterified)", "loq": 0.2, "labMethodLink": "" }, { "subSampleId": 2003368, "nutrientId": 1286, "nutrientAcquisitionDetails": [ { "sampleUnitId": 1999639, "purchaseDate": "1/11/2021", "storeCity": "(Region)", "storeState": "West", "packerCity": "", "packerState": "" } ], "amount": 0.0, "labMethodTechnique": "Direct alkaline saponification, BSTFA/TMCS derivatization; GC-FID", "labMethodDescription": "Phyto- & Fungisterols", "labMethodOriginalDescription": "Phyto- & Fungisterols (direct saponification, free and esterified)", "loq": 0.2, "labMethodLink": "" }, { "subSampleId": 2003369, "nutrientId": 1286, "nutrientAcquisitionDetails": [ { "sampleUnitId": 1999641, "purchaseDate": "1/5/2021", "storeCity": "(Region)", "storeState": "Plains", "packerCity": "", "packerState": "" } ], "amount": 0.0, "labMethodTechnique": "Direct alkaline saponification, BSTFA/TMCS derivatization; GC-FID", "labMethodDescription": "Phyto- & Fungisterols", "labMethodOriginalDescription": "Phyto- & Fungisterols (direct saponification, free and esterified)", "loq": 0.2, "labMethodLink": "" }, { "subSampleId": 2003370, "nutrientId": 1286, "nutrientAcquisitionDetails": [ { "sampleUnitId": 1999643, "purchaseDate": "1/5/2021", "storeCity": "(Region)", "storeState": "Plains", "packerCity": "", "packerState": "" } ], "amount": 0.0, "labMethodTechnique": "Direct alkaline saponification, BSTFA/TMCS derivatization; GC-FID", "labMethodDescription": "Phyto- & Fungisterols", "labMethodOriginalDescription": "Phyto- & Fungisterols (direct saponification, free and esterified)", "loq": 0.2, "labMethodLink": "" }, { "subSampleId": 2003371, "nutrientId": 1286, "nutrientAcquisitionDetails": [ { "sampleUnitId": 1999645, "purchaseDate": "1/19/2021", "storeCity": "Blacksburg", "storeState": "VA", "packerCity": "", "packerState": "" } ], "amount": 0.0, "labMethodTechnique": "Direct alkaline saponification, BSTFA/TMCS derivatization; GC-FID", "labMethodDescription": "Phyto- & Fungisterols", "labMethodOriginalDescription": "Phyto- & Fungisterols (direct saponification, free and esterified)", "loq": 0.2, "labMethodLink": "" } ], "loq": 0.2 }, { "type": "FoodNutrient", "nutrient": { "id": 1288, "number": "641", "name": "Beta-sitosterol", "rank": 16200, "unitName": "mg" }, "foodNutrientDerivation": { "id": 1, "code": "A", "description": "Analytical", "foodNutrientSource": { "id": 1, "code": "1", "description": "Analytical or derived from analytical" } }, "id": 24469944, "amount": 0.0, "dataPoints": 6, "max": 0.0, "min": 0.0, "median": 0.0, "minYearAcquired": 2021, "nutrientAnalysisDetails": [ { "subSampleId": 2003336, "nutrientId": 1288, "nutrientAcquisitionDetails": [ { "sampleUnitId": 1999635, "purchaseDate": "1/5/2021", "storeCity": "(Region)", "storeState": "Atlantic", "packerCity": "", "packerState": "" } ], "amount": 0.0, "labMethodTechnique": "Direct alkaline saponification, BSTFA/TMCS derivatization; GC-FID", "labMethodDescription": "Phyto- & Fungisterols", "labMethodOriginalDescription": "Phyto- & Fungisterols (direct saponification, free and esterified)", "loq": 0.2, "labMethodLink": "" }, { "subSampleId": 2003337, "nutrientId": 1288, "nutrientAcquisitionDetails": [ { "sampleUnitId": 1999637, "purchaseDate": "1/5/2021", "storeCity": "(Region)", "storeState": "Atlantic", "packerCity": "", "packerState": "" } ], "amount": 0.0, "labMethodTechnique": "Direct alkaline saponification, BSTFA/TMCS derivatization; GC-FID", "labMethodDescription": "Phyto- & Fungisterols", "labMethodOriginalDescription": "Phyto- & Fungisterols (direct saponification, free and esterified)", "loq": 0.2, "labMethodLink": "" }, { "subSampleId": 2003338, "nutrientId": 1288, "nutrientAcquisitionDetails": [ { "sampleUnitId": 1999639, "purchaseDate": "1/11/2021", "storeCity": "(Region)", "storeState": "West", "packerCity": "", "packerState": "" } ], "amount": 0.0, "labMethodTechnique": "Direct alkaline saponification, BSTFA/TMCS derivatization; GC-FID", "labMethodDescription": "Phyto- & Fungisterols", "labMethodOriginalDescription": "Phyto- & Fungisterols (direct saponification, free and esterified)", "loq": 0.2, "labMethodLink": "" }, { "subSampleId": 2003339, "nutrientId": 1288, "nutrientAcquisitionDetails": [ { "sampleUnitId": 1999641, "purchaseDate": "1/5/2021", "storeCity": "(Region)", "storeState": "Plains", "packerCity": "", "packerState": "" } ], "amount": 0.0, "labMethodTechnique": "Direct alkaline saponification, BSTFA/TMCS derivatization; GC-FID", "labMethodDescription": "Phyto- & Fungisterols", "labMethodOriginalDescription": "Phyto- & Fungisterols (direct saponification, free and esterified)", "loq": 0.2, "labMethodLink": "" }, { "subSampleId": 2003340, "nutrientId": 1288, "nutrientAcquisitionDetails": [ { "sampleUnitId": 1999643, "purchaseDate": "1/5/2021", "storeCity": "(Region)", "storeState": "Plains", "packerCity": "", "packerState": "" } ], "amount": 0.0, "labMethodTechnique": "Direct alkaline saponification, BSTFA/TMCS derivatization; GC-FID", "labMethodDescription": "Phyto- & Fungisterols", "labMethodOriginalDescription": "Phyto- & Fungisterols (direct saponification, free and esterified)", "loq": 0.2, "labMethodLink": "" }, { "subSampleId": 2003341, "nutrientId": 1288, "nutrientAcquisitionDetails": [ { "sampleUnitId": 1999645, "purchaseDate": "1/19/2021", "storeCity": "Blacksburg", "storeState": "VA", "packerCity": "", "packerState": "" } ], "amount": 0.0, "labMethodTechnique": "Direct alkaline saponification, BSTFA/TMCS derivatization; GC-FID", "labMethodDescription": "Phyto- & Fungisterols", "labMethodOriginalDescription": "Phyto- & Fungisterols (direct saponification, free and esterified)", "loq": 0.2, "labMethodLink": "" } ], "loq": 0.2 }, { "type": "FoodNutrient", "nutrient": { "id": 2060, "number": "", "name": "Ergosta-7-enol", "rank": 16210, "unitName": "mg" }, "foodNutrientDerivation": { "id": 1, "code": "A", "description": "Analytical", "foodNutrientSource": { "id": 1, "code": "1", "description": "Analytical or derived from analytical" } }, "id": 24469948, "amount": 1.634, "dataPoints": 6, "max": 1.93, "min": 1.365, "median": 1.635, "minYearAcquired": 2021, "nutrientAnalysisDetails": [ { "subSampleId": 2003186, "nutrientId": 2060, "nutrientAcquisitionDetails": [ { "sampleUnitId": 1999635, "purchaseDate": "1/5/2021", "storeCity": "(Region)", "storeState": "Atlantic", "packerCity": "", "packerState": "" } ], "amount": 1.75, "labMethodTechnique": "Direct alkaline saponification, BSTFA/TMCS derivatization; GC-FID", "labMethodDescription": "Phyto- & Fungisterols", "labMethodOriginalDescription": "Phyto- & Fungisterols (direct saponification, free and esterified)", "labMethodLink": "" }, { "subSampleId": 2003187, "nutrientId": 2060, "nutrientAcquisitionDetails": [ { "sampleUnitId": 1999637, "purchaseDate": "1/5/2021", "storeCity": "(Region)", "storeState": "Atlantic", "packerCity": "", "packerState": "" } ], "amount": 1.46, "labMethodTechnique": "Direct alkaline saponification, BSTFA/TMCS derivatization; GC-FID", "labMethodDescription": "Phyto- & Fungisterols", "labMethodOriginalDescription": "Phyto- & Fungisterols (direct saponification, free and esterified)", "labMethodLink": "" }, { "subSampleId": 2003188, "nutrientId": 2060, "nutrientAcquisitionDetails": [ { "sampleUnitId": 1999639, "purchaseDate": "1/11/2021", "storeCity": "(Region)", "storeState": "West", "packerCity": "", "packerState": "" } ], "amount": 1.365, "labMethodTechnique": "Direct alkaline saponification, BSTFA/TMCS derivatization; GC-FID", "labMethodDescription": "Phyto- & Fungisterols", "labMethodOriginalDescription": "Phyto- & Fungisterols (direct saponification, free and esterified)", "labMethodLink": "" }, { "subSampleId": 2003189, "nutrientId": 2060, "nutrientAcquisitionDetails": [ { "sampleUnitId": 1999641, "purchaseDate": "1/5/2021", "storeCity": "(Region)", "storeState": "Plains", "packerCity": "", "packerState": "" } ], "amount": 1.93, "labMethodTechnique": "Direct alkaline saponification, BSTFA/TMCS derivatization; GC-FID", "labMethodDescription": "Phyto- & Fungisterols", "labMethodOriginalDescription": "Phyto- & Fungisterols (direct saponification, free and esterified)", "labMethodLink": "" }, { "subSampleId": 2003190, "nutrientId": 2060, "nutrientAcquisitionDetails": [ { "sampleUnitId": 1999643, "purchaseDate": "1/5/2021", "storeCity": "(Region)", "storeState": "Plains", "packerCity": "", "packerState": "" } ], "amount": 1.78, "labMethodTechnique": "Direct alkaline saponification, BSTFA/TMCS derivatization; GC-FID", "labMethodDescription": "Phyto- & Fungisterols", "labMethodOriginalDescription": "Phyto- & Fungisterols (direct saponification, free and esterified)", "labMethodLink": "" }, { "subSampleId": 2003191, "nutrientId": 2060, "nutrientAcquisitionDetails": [ { "sampleUnitId": 1999645, "purchaseDate": "1/19/2021", "storeCity": "Blacksburg", "storeState": "VA", "packerCity": "", "packerState": "" } ], "amount": 1.52, "labMethodTechnique": "Direct alkaline saponification, BSTFA/TMCS derivatization; GC-FID", "labMethodDescription": "Phyto- & Fungisterols", "labMethodOriginalDescription": "Phyto- & Fungisterols (direct saponification, free and esterified)", "labMethodLink": "" } ] }, { "type": "FoodNutrient", "nutrient": { "id": 2062, "number": "", "name": "Ergosta-5,7-dienol", "rank": 16211, "unitName": "mg" }, "foodNutrientDerivation": { "id": 1, "code": "A", "description": "Analytical", "foodNutrientSource": { "id": 1, "code": "1", "description": "Analytical or derived from analytical" } }, "id": 24469952, "amount": 5.841, "dataPoints": 6, "max": 6.79, "min": 4.08, "median": 6.035, "minYearAcquired": 2021, "nutrientAnalysisDetails": [ { "subSampleId": 2003126, "nutrientId": 2062, "nutrientAcquisitionDetails": [ { "sampleUnitId": 1999635, "purchaseDate": "1/5/2021", "storeCity": "(Region)", "storeState": "Atlantic", "packerCity": "", "packerState": "" } ], "amount": 6.53, "labMethodTechnique": "Direct alkaline saponification, BSTFA/TMCS derivatization; GC-FID", "labMethodDescription": "Phyto- & Fungisterols", "labMethodOriginalDescription": "Phyto- & Fungisterols (direct saponification, free and esterified)", "labMethodLink": "" }, { "subSampleId": 2003127, "nutrientId": 2062, "nutrientAcquisitionDetails": [ { "sampleUnitId": 1999637, "purchaseDate": "1/5/2021", "storeCity": "(Region)", "storeState": "Atlantic", "packerCity": "", "packerState": "" } ], "amount": 6.38, "labMethodTechnique": "Direct alkaline saponification, BSTFA/TMCS derivatization; GC-FID", "labMethodDescription": "Phyto- & Fungisterols", "labMethodOriginalDescription": "Phyto- & Fungisterols (direct saponification, free and esterified)", "labMethodLink": "" }, { "subSampleId": 2003128, "nutrientId": 2062, "nutrientAcquisitionDetails": [ { "sampleUnitId": 1999639, "purchaseDate": "1/11/2021", "storeCity": "(Region)", "storeState": "West", "packerCity": "", "packerState": "" } ], "amount": 5.575, "labMethodTechnique": "Direct alkaline saponification, BSTFA/TMCS derivatization; GC-FID", "labMethodDescription": "Phyto- & Fungisterols", "labMethodOriginalDescription": "Phyto- & Fungisterols (direct saponification, free and esterified)", "labMethodLink": "" }, { "subSampleId": 2003129, "nutrientId": 2062, "nutrientAcquisitionDetails": [ { "sampleUnitId": 1999641, "purchaseDate": "1/5/2021", "storeCity": "(Region)", "storeState": "Plains", "packerCity": "", "packerState": "" } ], "amount": 4.08, "labMethodTechnique": "Direct alkaline saponification, BSTFA/TMCS derivatization; GC-FID", "labMethodDescription": "Phyto- & Fungisterols", "labMethodOriginalDescription": "Phyto- & Fungisterols (direct saponification, free and esterified)", "labMethodLink": "" }, { "subSampleId": 2003130, "nutrientId": 2062, "nutrientAcquisitionDetails": [ { "sampleUnitId": 1999643, "purchaseDate": "1/5/2021", "storeCity": "(Region)", "storeState": "Plains", "packerCity": "", "packerState": "" } ], "amount": 6.79, "labMethodTechnique": "Direct alkaline saponification, BSTFA/TMCS derivatization; GC-FID", "labMethodDescription": "Phyto- & Fungisterols", "labMethodOriginalDescription": "Phyto- & Fungisterols (direct saponification, free and esterified)", "labMethodLink": "" }, { "subSampleId": 2003131, "nutrientId": 2062, "nutrientAcquisitionDetails": [ { "sampleUnitId": 1999645, "purchaseDate": "1/19/2021", "storeCity": "Blacksburg", "storeState": "VA", "packerCity": "", "packerState": "" } ], "amount": 5.69, "labMethodTechnique": "Direct alkaline saponification, BSTFA/TMCS derivatization; GC-FID", 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